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Catalog Number	Size	Price
LS-B15709-50	50 µl (1 mg/ml)	$460

ADGRF5 / GPR116 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

ADGRF5 / GPR116 Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

ADGRF5 / GPR116 Antibody - Immunohistochemical analysis of GPR116 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

ADGRF5 / GPR116 Antibody - Immunofluorescent analysis of GPR116 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

ADGRF5 / GPR116 Antibody - Western blot analysis of GPR116 expression in HeLa (A); Y79 (B) whole cell lysates.

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IHC‑plus™ Polyclonal Rabbit anti‑Human ADGRF5 / GPR116 Antibody (N‑Terminus, IHC, IF, WB) LS‑B15709

Name: IHC-plus™ ADGRF5 / GPR116 Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B15709-50
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C359001

Antibody:

ADGRF5 / GPR116 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$460

LS-B15709-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ADGRF5 / GPR116 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

GPR116 antibody LS-B15709 is an unconjugated rabbit polyclonal antibody to human GPR116 (ADGRF5) (N-Terminus). Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human ADGRF5 / GPR116

Synonyms

See All ADGRF5 / GPR116 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human GPR116.

Epitope

N-Terminus

Specificity

Recognizes endogenous levels of GPR116 protein.

Applications

IHC
IHC - Paraffin (5 µg/ml)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ADGRF5 / GPR116

Uniprot: Q8IZF2 NCBI: NM_015234 AAN46672.1