Target
Mouse IgG1 + IgG2a + IgG2b + IgG3
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin,
FITC,
HRP,
TRITC.
Immunogen
Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Mouse IgG, heavy and light chains.
Usage
The reactivity of the antiserum is directed to the Fc and Fab subunits of IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against mouse serum a single characteristic precipitin line with a spur is usually obtained. It does not react with any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG. as secondary antibody to precipitate the immunoglobulin in normal mouse serum. to prepare an immunoadsorbent for the production of Ig-free mouse serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labeled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.
Presentation
Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee