Rat IgG1 + IgG2a + IgG2b + IgG2c

Goat

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Biotin. Also available Unconjugated or conjugated with HRP, TRITC, FITC.

Purified IgG

Unmodified

Purified normal IgG, including all known subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat IgG, heavy and light chains.

• IHC
• IHC - Paraffin
• ICC
• ELISA
• Dot Blot
• Immunodiffusion
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:10000.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.