Reactivity
Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Tetramethyl Rhodamine Iso-Thiocyanate.
Also available
Unconjugated or conjugated with
Biotin,
FITC,
HRP.
Purification
Purified IgG
Immunogen
Purified polyclonal IgG isolated from pooled Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Monkey IgG, Fc specific.
Applications
- IHC
- IHC - Frozen
- ICC
- Immunofluorescence
- ELISA
- Immunodiffusion
- Applications tested for the base form of this product only
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Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates. to demonstrate immunoglobulins or specific antibodies in cells and tissues. to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:30.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee