Reactivity
Pig
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
HRP.
Immunogen
Purified normal IgG isolated from pooled swine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Swine IgG, heavy and light chains.
Usage
The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. In immunoelectrophoresis against swine serum, a single characteristic precipitin line is usually observed, representing primarily Igg2. The IgG1 may become visible a spur. The antiserum includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against swine serum a single characteristic precipitin line with a spur is usually obtained. It does not react with any non-Ig protein in swine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG. as secondary antibody to precipitate the immunoglobulin in normal swine serum. to prepare an immunoadsorbent for the production of Ig-free swine serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labeled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.
Presentation
Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee