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Vector Laboratories, Inc.
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Customer Service: (800) 227-6666 / (650) 697-3600


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Catalog Number Size Price
LS-C662128-10 10 µg $318 
LS-C662128-100 100 µg $470 
IGFBP5 Antibody - Western blot analysis of IGFBP5 using anti-IGFBP5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP5 at approximately 31KD. The expected band size for IGFBP5 is at 31KD.

Polyclonal Rabbit anti‑Human IGFBP5 Antibody (WB) LS‑C662128

Polyclonal Rabbit anti‑Human IGFBP5 Antibody (WB) LS‑C662128

Antibody:
IGFBP5 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662128-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IGFBP5 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IGFBP5 antibody LS-C662128 is an unconjugated rabbit polyclonal antibody to human IGFBP5. Validated for ELISA and WB.
Target
Human IGFBP5
Synonyms
IGFBP5 | IBP-5 | IGFBP-5 | IBP5 | IGF-binding protein 5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human IGFBP5 recombinant protein (Position: A60-E272). Human IGFBP5 shares 96.7% and 96.2% amino acid (aa) sequence identity with mouse and rat IGFBP5, respectively.
Specificity
Osteosarcoma, and at lower levels in liver, kidney and brain.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IGFBP5
P24593 NM_000599 NP_000590.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024