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Antibodies > IGFBP2 / IGF-BP53 > LS-C662087
 
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Catalog Number Size Price
LS-C662087-10 10 µg $318 
LS-C662087-100 100 µg $470 
IGFBP2 / IGF-BP53 Antibody - Immunohistochemistry - Anti-IGFBP2 Picoband Antibody
IGFBP2 / IGF-BP53 Antibody - Immunohistochemistry - Anti-IGFBP2 Picoband Antibody
IGFBP2 / IGF-BP53 Antibody - Western blot analysis of IGFBP2 using anti-IGFBP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 35KD. The expected band size for IGFBP2 is at 35KD.
IGFBP2 / IGF-BP53 Antibody - Western blot - Anti-IGFBP2 Picoband Antibody
IGFBP2 / IGF-BP53 Antibody - Immunohistochemistry - Anti-IGFBP2 Picoband Antibody
IGFBP2 / IGF-BP53 Antibody - Immunohistochemistry - Anti-IGFBP2 Picoband Antibody
IGFBP2 / IGF-BP53 Antibody - Western blot analysis of IGFBP2 using anti-IGFBP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 35KD. The expected band size for IGFBP2 is at 35KD.
IGFBP2 / IGF-BP53 Antibody - Western blot - Anti-IGFBP2 Picoband Antibody
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Polyclonal Rabbit anti‑Human IGFBP2 / IGF‑BP53 Antibody (IHC, WB) LS‑C662087

Polyclonal Rabbit anti‑Human IGFBP2 / IGF‑BP53 Antibody (IHC, WB) LS‑C662087

Submit Review LSBio Guarantee
Antibody:
IGFBP2 / IGF-BP53 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662087-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IGFBP2 / IGF-BP53 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IGF-BP53 antibody LS-C662087 is an unconjugated rabbit polyclonal antibody to human IGF-BP53 (IGFBP2). Validated for ELISA, IHC and WB.
Target
Human IGFBP2 / IGF-BP53
Synonyms
IGFBP2 | BP2 | IGFBP-2 | IGF-binding protein 2 | IBP-2 | IBP2 | IGF-BP53
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human IGFBP2 recombinant protein (Position: A36-Q325).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IGFBP2 / IGF-BP53
P18065 NM_000597 NP_000588.2

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IHC‑plus™ IGFBP2 / IGF‑BP53 Antibody (aa277‑305) LS‑B9078
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
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IGFBP2 / IGF‑BP53 Antibody LS‑C36898
Species: Bovine, Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation, ELISA
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IGFBP2 / IGF‑BP53 Antibody LS‑C7524
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Applications: Western blot, ELISA
IGFBP2 / IGF-BP53 Antibody - IHC of paraffin-embedded human lung cancer (A), recturn(B), prostate (C), colon cancer (D) showing cytoplasmic localization using IGFBP2 mouse monoclonal antibody with DAB staining.
IGFBP2 / IGF‑BP53 Antibody (clone 1F6F6) LS‑C108883
Species: Human
Applications: IHC, Western blot, ELISA
IGFBP2 / IGF-BP53 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP2.
IGFBP2 / IGF‑BP53 Antibody (clone OTI1A7) LS‑C173803
Species: Human
Applications: Western blot, Flow Cytometry
IGFBP2 / IGF-BP53 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
IGFBP2 / IGF‑BP53 Antibody (aa215‑264) LS‑B11951
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
See all IGFBP2 / IGF-BP53 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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