Bovine IgA, Secretory

Rabbit

Bovine (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

Highly purified secretory IgA isolated from pooled bovine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Bovine secretory IgA.

• Immunodiffusion

The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and to surface and hidden determinants of the secretory component as tested in immunoelectrophoresis and radial immunodiffusion. In immunoelectrophoresis using various antiserum concentrations against normal bovine milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal bovine serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in bovine serum, milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of bovine IgA from serum or exocrine secretions. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include.

Delipidated, heat-inactivated, lyophilized antiserum. No preservative.

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.