Target
Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase.
Also available
Unconjugated or conjugated with
FITC,
TRITC,
Biotin.
Purification
Purified IgG
Immunogen
Purified polyclonal rat IgG, and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Rat IgG, IgA and IgM, Fc specific.
Applications
- IHC
- IHC - Paraffin
- ICC
- ELISA
- Dot Blot
- Applications tested for the base form of this product only
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Usage
The reactivity of the antiserum is directed to the Fc subunit of the major immunoglobulin isotypes (classes and subclasses) which expresses strict isotypic (class) specificity. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bound to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee