Target
Human IgA + IgD + IgG + IgM
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified IgG
Immunogen
Highly purified IgG, and pools of homogenous IgA, IgM and IgD isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Human immunoglobulins IgG, IgA, IgM and IgD, heavy and light chains.
Applications
- Immunofluorescence
- ELISA
- Dot Blot
Usage
The defined antibody specificity is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibody to common determinants and to class specific determinants and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA, IgM and IgD (if present in precipitable amounts) precipitin lines are obtained. The cytochemical grade of this antibodyS allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates. in radioimmunoassay. for the production of immunoconjugates with a selected marker. to prepare immunoaffinity adsorbents by coupling to an artificial carrier. in non-isotopic methodology based on solid phase immunochemistry (e. g. ELISA), both as catching antibody and detection reagent. in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays are between 1:1000 and 1:5000.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee