Target
Mouse Ig Kappa Light Chain
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
FITC.
Also available
Unconjugated or conjugated with
TRITC.
Purification
Purified IgG
Immunogen
A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Free and bound Mouse Ig kappa light chain.
Applications
- IHC
- IHC - Frozen
- ICC
- Immunofluorescence
- ELISA
- Applications tested for the base form of this product only
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Usage
The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of mouse serum or plasma. This conjugate is intended for use in direct immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the kappa type as well as free light chains or Bence Jones proteins of the kappa type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee