The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human IFN-beta by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-beta. To detect Human IFN-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IFN-beta (
LS-C104330) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-beta. Western Blot: To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.