Target
Human IDH1 / IDH
Synonyms
IDH1 | IDCD | IDP | IDPC | PICD | NADP(+)-specific ICDH | IDH | Oxalosuccinate decarboxylase
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence KKHNVGVKCATIT, from the internal region of the protein sequence according to NP_005887.2.
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Usage
Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 40+45kD bands observed in lysates of cell line HepG2 (calculated MW of 46.7kD according to NP_005887.2). The observed 40kD is consistent with observaton with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee