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Catalog Number	Size	Price
LS-C407813-10	10 µg	$318
LS-C407813-100	100 µg	$470

IDH1 / IDH Antibody - IHC analysis of IDH1 using anti-IDH1 antibody. IDH1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IDH1 / IDH Antibody - IHC analysis of IDH1 using anti-IDH1 antibody. IDH1 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IDH1 / IDH Antibody - IDH1 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.

IDH1 / IDH Antibody - IDH1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.

IDH1 / IDH Antibody - IDH1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

IDH1 / IDH Antibody - IDH1 antibody Western blot. All lanes: Anti IDH1 at 0.5 ug/ml. Lane 1: Rat Lung Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: Rat Brain Tissue Lysate at 50 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: SMMC Whole Cell Lysate at 40 ug. Lane 6: A549 Whole Cell Lysate at 40 ug. Lane 7: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 47 kD. Observed band size: 47 kD.

IDH1 / IDH Antibody - Flow Cytometry analysis of HepG2 cells using anti-IDH1 antibody. Overlay histogram showing HepG2 cells stained with anti-IDH1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDH1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human IDH1 / IDH Antibody (aa381‑413, IHC, WB) LS‑C407813

Name: Anti-IDH1 / IDH Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407813-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

IDH1 / IDH Rabbit anti-Human Polyclonal (aa381-413) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat, Bat, Pig

Format:

Unconjugated, Unmodified

$318

LS-C407813-10

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For Research Use Only

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Overview

Antibody:

IDH1 / IDH Rabbit anti-Human Polyclonal (aa381-413) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat, Bat, Pig

Format:

Unconjugated, Unmodified

Specifications

Description

IDH antibody LS-C407813 is an unconjugated rabbit polyclonal antibody to IDH (IDH1) (aa381-413) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.

Target

Human IDH1 / IDH

Synonyms

See All IDH1 / IDH Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat, Bat, Pig (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human IDH1 (381-413 aa KGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAK), different from the related mouse and rat sequences by one amino acid.

Epitope

aa381-413

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IDH1 / IDH

Uniprot: O75874 NCBI: NM_005896 NP_005887.2