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Catalog Number Size Price
LS-C777335-100 100 µg (1 mg/ml) $478 
HYOU1 / ORP150 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GRP170 Antibody. (D) Composite.
HYOU1 / ORP150 Antibody - Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysates showing detection of ~170 kDa GRP170 protein using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK-293 cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.
HYOU1 / ORP150 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GRP170 Antibody. (D) Composite.
HYOU1 / ORP150 Antibody - Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysates showing detection of ~170 kDa GRP170 protein using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK-293 cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.
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Monoclonal Mouse anti‑Human HYOU1 / ORP150 Antibody (clone 6G7‑2H5, APC, IF, WB) LS‑C777335

Monoclonal Mouse anti‑Human HYOU1 / ORP150 Antibody (clone 6G7‑2H5, APC, IF, WB) LS‑C777335

Antibody:
HYOU1 / ORP150 Mouse anti-Human Monoclonal (APC) (6G7-2H5) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Allophycocyanin, Unmodified
Other formats:
Price
Catalog Number
$478
LS-C777335-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HYOU1 / ORP150 Mouse anti-Human Monoclonal (APC) (6G7-2H5) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Allophycocyanin, Unmodified
Other formats:

Specifications

Description
ORP150 antibody LS-C777335 is an APC-conjugated mouse monoclonal antibody to human ORP150 (HYOU1). Validated for IF and WB.
Target
Human HYOU1 / ORP150
Synonyms
HYOU1 | GRP-170 | Grp170 | Glucose-regulated protein 170 | Hypoxia up-regulated 1 | Hypoxia up-regulated protein 1 | HSP12A | ORP150 | ORP-150
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
6G7-2H5
Conjugations
Allophycocyanin. Also available conjugated with Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Raised against a synthetic peptide of human GRP170
Specificity
Detects ~170 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HYOU1 / ORP150
Q9Y4L1 NM_006389 NP_006380.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/27/2024