Rat HTR7 / 5HT7 Receptor

Rabbit

Rat, Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide sequence corresponding to aa 8-23 of the rat 5-HT7 receptor.

aa8-23

Recognizes Serotonin Receptor 7. Species cross-reactivity: Mouse and rat.

• IHC (1:100 - 1:300)
• Immunofluorescence
• Western blot (1:100 - 1:250)

Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100-1:250. Immunohistochemistry: 1:100-1:300 in PBS - Bn/Av-HRP Technique. Control Tissue: The antiserum demonstrates positive labeling of rat cortex and. hippocampus. Perfusion Fixation: Fixative-4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over approximately 20 minutes. Post Fixation: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over approximately 20 minutes. Post Fixation-1.5 hour at 4? C. in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.10.3%) may be used in conjunction with paraformaldehyde. Sections: 50um vibratome. Antibody dilution: 1:100-1:300 in PBS-Bn-SA/HRP Technique. Absorption control: Synthetic rat 5HT 7 receptor. Incubation on Tissue: 16-24 hours at 4?C.

Supplied as a liquid

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P34969 NCBI: NM_019859 NP_062873.1