Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (clone HSPB1/774, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C390379
Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (clone HSPB1/774, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C390379
Antibody:
HSPB1 / HSP27 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (HSPB1/774) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Chimpanzee, Mouse, Rat, Sheep, Chicken
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
HSPB1 / HSP27 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (HSPB1/774) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Chimpanzee, Mouse, Rat, Sheep, Chicken
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
HSP27 antibody LS-C390379 is an unconjugated mouse monoclonal antibody to HSP27 (HSPB1) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Reactivity
Human, Chimpanzee, Mouse, Rat, Sheep, Chicken
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified or
Ready-to-Use.
Immunogen
Recombinant human HSPB1 protein.
Specificity
Human HSPB1 / HSP27
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (0.5 - 1 µg/ml)
- Western blot (0.25 - 0.5 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells. Optimal dilution of the HSP27 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Turkey, Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Species:
Human, Monkey, Bovine, Guinea pig, Pig
Applications:
Western blot
Species:
Human, Chimpanzee, Mouse, Rat, Sheep, Chicken
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 10/19/2024