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Antibodies > HSPA2 > LS-C407851
 
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Catalog Number Size Price
LS-C407851-10 10 µg $318 
LS-C407851-100 100 µg $470 
HSPA2 Antibody - HSPA2 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
HSPA2 Antibody - HSPA2 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
HSPA2 Antibody - HSPA2 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
HSPA2 Antibody - IF analysis of HSPA2 using anti-HSPA2 antibody HSPA2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HSPA2 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HSPA2 Antibody - IF analysis of HSPA2 using anti-HSPA2 antibody HSPA2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HSPA2 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HSPA2 Antibody - HSPA2 antibody Western blot. All lanes: Anti HSPA2 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Thymus Tissue Lysate at 50 ug. Lane 3: Rat Testis Tissue Lysate at 50 ug. Lane 4: Mouse Liver Tissue Lysate at 50 ug. Lane 5: Mouse Kidney Tissue Lysate at 50 ug. Lane 6: HELA Whole Cell Lysate at 40 ug. Lane 7: MCF-7 Whole Cell Lysate at 40 ug. Lane 8: A375 Whole Cell Lysate at 40 ug. Lane 9: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 70 kD. Observed band size: 70 kD.
HSPA2 Antibody - Flow Cytometry analysis of PC-3 cells using anti-HSPA2 antibody. Overlay histogram showing PC-3 cells stained with anti-HSPA2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPA2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
HSPA2 Antibody - HSPA2 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
HSPA2 Antibody - HSPA2 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
HSPA2 Antibody - HSPA2 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
HSPA2 Antibody - IF analysis of HSPA2 using anti-HSPA2 antibody HSPA2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HSPA2 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HSPA2 Antibody - IF analysis of HSPA2 using anti-HSPA2 antibody HSPA2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HSPA2 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HSPA2 Antibody - HSPA2 antibody Western blot. All lanes: Anti HSPA2 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Thymus Tissue Lysate at 50 ug. Lane 3: Rat Testis Tissue Lysate at 50 ug. Lane 4: Mouse Liver Tissue Lysate at 50 ug. Lane 5: Mouse Kidney Tissue Lysate at 50 ug. Lane 6: HELA Whole Cell Lysate at 40 ug. Lane 7: MCF-7 Whole Cell Lysate at 40 ug. Lane 8: A375 Whole Cell Lysate at 40 ug. Lane 9: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 70 kD. Observed band size: 70 kD.
HSPA2 Antibody - Flow Cytometry analysis of PC-3 cells using anti-HSPA2 antibody. Overlay histogram showing PC-3 cells stained with anti-HSPA2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPA2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human HSPA2 Antibody (aa564‑598, IHC, WB) LS‑C407851

Polyclonal Rabbit anti‑Human HSPA2 Antibody (aa564‑598, IHC, WB) LS‑C407851

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Antibody:
HSPA2 Rabbit anti-Human Polyclonal (aa564-598) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Dog, Hamster, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407851-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HSPA2 Rabbit anti-Human Polyclonal (aa564-598) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Dog, Hamster, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
HSPA2 antibody LS-C407851 is an unconjugated rabbit polyclonal antibody to HSPA2 (aa564-598) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human HSPA2
Synonyms
HSPA2 | Heat shock 70kD protein 2 | HSP70-2 | HSP70-3 | Heat shock 70 kDa protein 2 | Heat shock 70kDa protein 2
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Hamster, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human HSPA2 (564-598 aa KISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHK), identical to the related mouse and rat sequences.
Epitope
aa564-598
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA2
P54652 NM_021979 NP_068814.2

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Featured Products

HSPA2 Antibody - Western blot analysis of HSPA2 using anti-HSPA2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen Tissue Lysate, Lane 2: mouse testis tissue Lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPA2 at approximately 70KD. The expected band size for HSPA2 is at 70KD.
HSPA2 Antibody (aa580‑599) LS‑C312957
Species: Human, Monkey, Mouse, Rat, Bovine, Goat, Pig, Sheep
Applications: Western blot
HSPA2 ELISA Kit
Human HSPA2 (Sandwich ELISA) ELISA Kit - LS-F35944
Reactivity: Human
Range: 0.78-50 ng/ml
HSPA2 Protein
Human HSPA2 Protein (Recombinant His, N-Terminal) (Ser2-Asp639) - LS-G97005
Source: E. coli
Tag: His, N-Terminal
See all HSPA2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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For technical assistance:
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