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Antibodies > HSF1 > LS-C356117
 
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Catalog Number Size Price
LS-C356117-30 30 µl $263 
LS-C356117-50 50 µl $279 
LS-C356117-100 100 µl (1 mg/ml) $315 
LS-C356117-200 200 µl $379 
HSF1 Antibody - Immunohistochemical analysis of HSF1 (pS303) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HSF1 Antibody - Immunofluorescent analysis of HSF1 (pS303) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSF1 Antibody - Western blot analysis of HSF1 (pS303) expression in HeLa (A); COLO205 (B) whole cell lysates.
HSF1 Antibody - Immunohistochemical analysis of HSF1 (pS303) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HSF1 Antibody - Immunofluorescent analysis of HSF1 (pS303) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSF1 Antibody - Western blot analysis of HSF1 (pS303) expression in HeLa (A); COLO205 (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human HSF1 Antibody (phospho‑Ser303, IHC, IF, WB) LS‑C356117

Polyclonal Rabbit anti‑Human HSF1 Antibody (phospho‑Ser303, IHC, IF, WB) LS‑C356117

Submit Review LSBio Guarantee
Antibody:
HSF1 Rabbit anti-Human Polyclonal (pSer303) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C356117-30
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HSF1 Rabbit anti-Human Polyclonal (pSer303) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
HSF1 antibody LS-C356117 is an unconjugated rabbit polyclonal antibody to HSF1 (pSer303) from human. It is reactive with human, bovine and monkey. Validated for ICC, IF, IHC and WB.
Target
Human HSF1
Synonyms
HSF1 | HSTF 1 | Heat shock factor protein 1 | HSF 1 | HSTF1
Host
Rabbit
Reactivity
Human, Monkey, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human HSF1 (pS303).
Epitope
pSer303
Specificity
Recognizes endogenous levels of HSF1 (pS303) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSF1
Q00613 NM_005526 NP_005517.1

Publications (0)

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/5/2025
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