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Antibodies > HSD17B10 / HADH2 > LS-C357522
 
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Catalog Number Size Price
LS-C357522-10 10 µg $318 
LS-C357522-100 100 µg (0.5 mg/ml) $470 
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - ERAB antibody IHC-paraffin: Human Mammary Cancer Tissue.
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - ERAB antibody Western blot. All lanes: Anti ERAB at 0.5 ug/ml. Lane 1: Mouse Lung Tissue Lysate at 50 ug. Lane 2: U87 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Lane 4: SW620 Whole Cell Lysate at 40 ug. Lane 5: 293T Whole Cell Lysate at 40 ug. Predicted band size: 27 kD. Observed band size: 27 kD.
HSD17B10 / HADH2 Antibody - Flow Cytometry analysis of A431 cells using anti-ERAB antibody. Overlay histogram showing A431 cells stained with anti-ERAB antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERAB Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
HSD17B10 / HADH2 Antibody - Flow Cytometry analysis of A549 cells using anti-ERAB antibody. Overlay histogram showing A549 cells stained with anti-ERAB antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERAB Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - ERAB antibody IHC-paraffin: Human Mammary Cancer Tissue.
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERAB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
HSD17B10 / HADH2 Antibody - ERAB antibody Western blot. All lanes: Anti ERAB at 0.5 ug/ml. Lane 1: Mouse Lung Tissue Lysate at 50 ug. Lane 2: U87 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Lane 4: SW620 Whole Cell Lysate at 40 ug. Lane 5: 293T Whole Cell Lysate at 40 ug. Predicted band size: 27 kD. Observed band size: 27 kD.
HSD17B10 / HADH2 Antibody - Flow Cytometry analysis of A431 cells using anti-ERAB antibody. Overlay histogram showing A431 cells stained with anti-ERAB antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERAB Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
HSD17B10 / HADH2 Antibody - Flow Cytometry analysis of A549 cells using anti-ERAB antibody. Overlay histogram showing A549 cells stained with anti-ERAB antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERAB Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human HSD17B10 / HADH2 Antibody (aa48‑261, IHC, WB) LS‑C357522

Polyclonal Rabbit anti‑Human HSD17B10 / HADH2 Antibody (aa48‑261, IHC, WB) LS‑C357522

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C388413
Antibody:
HSD17B10 / HADH2 Rabbit anti-Human Polyclonal (aa48-261) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357522-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HSD17B10 / HADH2 Rabbit anti-Human Polyclonal (aa48-261) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
HADH2 antibody LS-C357522 is an unconjugated rabbit polyclonal antibody to HADH2 (HSD17B10) (aa48-261) from human. It is reactive with human and mouse. Validated for IHC and WB.
Target
Human HSD17B10 / HADH2
Synonyms
HSD17B10 | 17-beta-HSD 10 | 17b-HSD10 | CAMR | DUPXp11.22 | ABAD | ERAB | HADH2 | MHBD | MRPP2 | MRX17 | SCHAD | SDR5C1 | MRX31 | MRXS10 | Type II HADH | HCD2 | XH98G2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human ERAB recombinant protein (Position: E48-P261). Human ERAB shares 87% and 88% amino acid (aa) sequence identity with mouse and rat ERAB, respectively.
Epitope
aa48-261
Specificity
Ubiquitously expressed in normal tissues but is overexpressed in neurons affected in AD. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSD17B10 / HADH2
Q99714 NM_004493 NP_004484.1

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Featured Products

HSD17B10 / HADH2 Antibody - IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSD17B10 mouse monoclonal antibody.
HSD17B10 / HADH2 Antibody (clone OTI11A2) LS‑C114806
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
HSD17B10 / HADH2 Antibody - Anti-HSD17B10 / HADH2 antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ HSD17B10 / HADH2 Antibody (aa242‑252) LS‑B9190
Species: Human, Mouse, Rat, Bovine, Hamster, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
HSD17B10 / HADH2 Antibody - Immunohistochemical analysis of HADH2 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HSD17B10 / HADH2 Antibody (Internal) LS‑C352301
Species: Human, Mouse, Rat, Bovine, Dog, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
HSD17B10 / HADH2 Antibody - Western blot. All lanes: 3-hydroxyacyl-CoA dehydrogenase type-2 antibody at 2 ug/ml Lane 1:mouse brain tissue Lane 2: 293T whole cell lysate. Lane 3: HepG2 whole cell lysate Lane 4: HeLa whole cell lysate Lane 5: HT29 whole cell lysate. Secondary antibody: Goat polyclonal. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HSD17B10 / HADH2 Antibody (aa2‑261, HRP) LS‑C371996
Species: Human
Applications: Western blot, ELISA
HSD17B10 / HADH2 ELISA Kit
Human HSD17B10 / HADH2 (Sandwich ELISA) ELISA Kit - LS-F11610
Reactivity: Human
Range: 0.156-10 ng/ml
See all HSD17B10 / HADH2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/12/2025
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