Target
Mouse HSD11B1 / HSD11B
Synonyms
HSD11B1 | 11-beta-HSD1 | 11-DH | CORTRD2 | HSD11B | HSD11L | SDR26C1 | HDL | HSD11
Reactivity
Mouse, Rat, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-TARSEEGLQKVVSR, from the internal region of the protein sequence according to NP_032314.2.
Specificity
Mouse HSD11B1.
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 32kD band observed in Mouse Lung lysates (calculated MW of 32.4kD according to NP_032314.2). Recommended concentration: 0.3-1 ug/ml. An additional band of unknown identity was also consistently observed at 45kD. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee