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Catalog Number Size Price
LS-B1745-50 50 µg (0.5 mg/ml) $485 
HRH1 / Histamine H1 Receptor Antibody - Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC‑plus™ Polyclonal Goat anti‑Human HRH1 / Histamine H1 Receptor Antibody (aa471‑484, IHC, IF) LS‑B1745

IHC‑plus™ Polyclonal Goat anti‑Human HRH1 / Histamine H1 Receptor Antibody (aa471‑484, IHC, IF) LS‑B1745

Note: This antibody replaces LS-C47564, LS-C67886
Antibody:
HRH1 / Histamine H1 Receptor Goat anti-Human Polyclonal (aa471-484) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Bat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B1745-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HRH1 / Histamine H1 Receptor Goat anti-Human Polyclonal (aa471-484) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Bat
Format:
Unconjugated, Unmodified

Specifications

Description
Histamine H1 Receptor antibody LS-B1745 is an unconjugated goat polyclonal antibody to Histamine H1 Receptor (HRH1) (aa471-484) from human. It is reactive with human and bat. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human HRH1 / Histamine H1 Receptor
Synonyms
HRH1 | H1 histamine receptor | H1 receptor | Histamine 1 receptor | Histamine H1 receptor | H1-R | H1R | Histamine H(1) receptor | Histamine receptor H1 | HisH1
Host
Goat
Reactivity
Human, Bat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence CNENFKKTFKRILH, from the C-Terminus of protein sequence according to NP_000852.1NP_001091681.1NP_001091682.1NP_001091683.1.
Epitope
aa471-484
Specificity
Human HRH1 / Histamine H1 Receptor. Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1745 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1745 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HRH1 / Histamine H1 Receptor
P35367 NM_000861 NP_000852.1

Validation

HRH1 / Histamine H1 Receptor Antibody - Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ HRH1 / Histamine H1 Receptor Antibody (aa471-484) for IHC, IF/Immunofluorescence LS-B1745 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)

Publications (3)

Estrogenic regulation of histamine receptor subtype H1 expression in the ventromedial nucleus of the hypothalamus in female rats. Mori H, Matsuda K, Yamawaki M, Kawata M. PloS one. 2014 9:e96232.

A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells. Uchida H, Machida M, Miura T, Kawasaki T, Okazaki T, Sasaki K, Sakamoto S, Ohuchi N, Kasahara M, Umezawa A, Akutsu H. JCI insight. 2017 2:e86492. (ICC; Human)

Histamine-dependent interactions between mast cells, glia, and neurons are altered following early-life adversity in mice and humans. Jonathon L McClain, Elvio A Mazzotta, Nidia Maradiaga, Natalia Duque-Wilckens, Iveta Grants, Alfred J Robison, Fievos L Christofi, Adam J Moeser, Brian D Gulbransen. American journal of physiology. Gastrointestinal and liver physiology. 2020 December;319:G655-G668.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/24/2024