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Catalog Number Size Price
LS-C662487-10 10 µg $318 
LS-C662487-100 100 µg $470 
HLA-DQB1 Antibody - Western blot analysis of HLA-DQB1 using anti-HLA-DQB1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DQB1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HLA-DQB1 at approximately 30KD. The expected band size for HLA-DQB1 is at 30KD.
HLA-DQB1 Antibody - Western blot - Anti-HLA-DQB1/Hla Dqb1 Picoband antibody
HLA-DQB1 Antibody - Western blot analysis of HLA-DQB1 using anti-HLA-DQB1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DQB1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HLA-DQB1 at approximately 30KD. The expected band size for HLA-DQB1 is at 30KD.
HLA-DQB1 Antibody - Western blot - Anti-HLA-DQB1/Hla Dqb1 Picoband antibody
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Polyclonal Rabbit anti‑Human HLA‑DQB1 Antibody (WB) LS‑C662487

Polyclonal Rabbit anti‑Human HLA‑DQB1 Antibody (WB) LS‑C662487

Antibody:
HLA-DQB1 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662487-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HLA-DQB1 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HLA-DQB1 antibody LS-C662487 is an unconjugated rabbit polyclonal antibody to human HLA-DQB1. Validated for WB.
Target
Human HLA-DQB1
Synonyms
HLA-DQB1 | Lymphocyte antigen | MHC class II DQ beta chain | MHC class2 antigen | MHC class II antigen DQB1 | HLA-DQB | IDDM1 | CELIAC1 | MHC DQ beta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence of human HLA-DQB1 (DAEYWNSQKEVLERTRAELDTVCRHNYQLELRTTLQRR).
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HLA-DQB1
P01920

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024