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Antibodies > Histone H3 > LS-C413067
 
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Catalog Number Size Price
LS-C413067-100 100 µl (1 mg/ml) $321 
Histone H3 Antibody - Immunohistochemical analysis of Histone H3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Histone H3 Antibody - Immunofluorescent analysis of Histone H3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Histone H3 Antibody - Western blot analysis of Histone H3 expression in MCF7 (A); Raw264.7 (B); mouse heart (C); rat heart (D) whole cell lysates.
Histone H3 Antibody - Immunohistochemical analysis of Histone H3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Histone H3 Antibody - Immunofluorescent analysis of Histone H3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Histone H3 Antibody - Western blot analysis of Histone H3 expression in MCF7 (A); Raw264.7 (B); mouse heart (C); rat heart (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human Histone H3 Antibody (N‑Terminus, IHC, IF, WB) LS‑C413067

Polyclonal Rabbit anti‑Human Histone H3 Antibody (N‑Terminus, IHC, IF, WB) LS‑C413067

Submit Review LSBio Guarantee
Antibody:
Histone H3 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, Flo
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$321
LS-C413067-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Histone H3 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, Flo
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
Histone H3 antibody LS-C413067 is an unconjugated rabbit polyclonal antibody to Histone H3 (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, ICC, IF, IHC and WB.
Target
Human Histone H3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Histone H3.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of Histone H3 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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IHC‑plus™ Histone H3 Antibody LS‑B6334
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Applications: IHC, Western blot
Histone H3 Antibody - Anti-Histone H3 antibody IHC of human breast epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-7.5 ug/ml.
IHC‑plus™ Histone H3 Antibody (Acetyl‑Lys18) LS‑B8486
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Histone H3 Antibody - Immunohistochemical analysis of Histone H3 (pS10) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Histone H3 Antibody (phospho‑Ser10) LS‑C353149
Species: Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all Histone H3 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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