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Catalog Number	Size	Price
LS-C676835-50	50 µl	$298
LS-C676835-100	100 µl	$370

HIST1H1C Antibody - Immunocytochemistry analysis of Crotonyl-HIST1H1C (K158) Antibody diluted at 1:50 and staining in Hela cells (treated with 30mM sodium crotonylate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

HIST1H1C Antibody - Immunofluorescent analysis of Hela cells (treated with 30mM crotonylate for 4h) diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

HIST1H1C Antibody - Chromatin Immunoprecipitation Hela (4*10E6, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1c (Crotonyl-HIST1H1C (K158) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.

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Polyclonal Rabbit anti‑Human HIST1H1C Antibody (IF) LS‑C676835

Name: Anti-HIST1H1C Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C676835-50
Price: 298.0000 USD
Availability: InStock