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| Catalog Number | Size | Price |
|---|---|---|
| LS-C67894-100 | 100 µg | $832 |
Monoclonal Mouse His Tag Antibody (IF, WB) LS‑C67894
Monoclonal Mouse His Tag Antibody (IF, WB) LS‑C67894
Antibody:
His Tag Mouse Monoclonal Antibody
Application:
IF, WB, IP, Flo, ELISA
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
His Tag Mouse Monoclonal Antibody
Application:
IF, WB, IP, Flo, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
His Tag antibody LS-C67894 is an unconjugated mouse monoclonal antibody to His Tag. Validated for ELISA, Flow, IF, IP and WB.
Host
Mouse
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide containing six histidine residues (6xHis) coupled to KLH.
Specificity
Recognizes C-terminal, N-terminal, and internal tagged fusion proteins.
Applications
- Immunofluorescence (1 µg/ml)
- Western blot (0.1 - 0.2 µg/ml)
- Immunoprecipitation (1 µg/ml)
- Flow Cytometry (1 µg/ml)
- ELISA (0.05 - 0.2 µg/ml)
Usage
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Immunofluorescence: 1 ug/ml. Flow Cytometry: 1 ug/ml. ELISA: 0.05-0.2 ug/ml. Western Blot: 0.1-0.2 ug/ml. Immunoprecipitation: 1 ug/ml.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide
Reconstitution
Reconstitute with 200ul sterile ddH2O
Storage
Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Synthetic
Applications:
Immunofluorescence, Flow Cytometry
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/his-tag-antibody-hrp-ls-c154274/81964_281469.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/6/2025
Date Requested: 2/6/2025
