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| Catalog Number | Size | Price |
|---|---|---|
| LS-C153999-100 | 100 µg (1.09 mg/ml) | $443 |
1 of 2
2 of 2
Monoclonal Mouse His Tag Antibody (clone 33D10.D2.G8, WB) LS‑C153999
Monoclonal Mouse His Tag Antibody (clone 33D10.D2.G8, WB) LS‑C153999
Antibody:
His Tag Mouse Monoclonal (33D10.D2.G8) Antibody
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
His Tag Mouse Monoclonal (33D10.D2.G8) Antibody
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
His Tag antibody LS-C153999 is an unconjugated mouse monoclonal antibody to His Tag. Validated for ELISA, IP and WB.
Host
Mouse
Clonality
IgG1,k
Monoclonal
Clone
33D10.D2.G8
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Specificity
This antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:200)
- ELISA (1:75000)
Usage
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Synthetic
Applications:
Immunofluorescence, Flow Cytometry
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/his-tag-antibody-hrp-ls-c154274/81964_281469.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/19/2024
Date Requested: 12/19/2024
