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Catalog Number Size Price
LS-C816499-100 100 µl $412 
HINT3 Antibody - Immunochemical staining of human HINT3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
HINT3 Antibody - Immunofluorescence staining of HINT3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HINT3 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.
HINT3 Antibody - Anti-HINT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa. (We are unsure as to the identity of these extra bands.)
HINT3 Antibody - HINT3 was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate. 4 uL anti-HINT3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-HINT3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa.
HINT3 Antibody - Immunochemical staining of human HINT3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
HINT3 Antibody - Immunofluorescence staining of HINT3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HINT3 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.
HINT3 Antibody - Anti-HINT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa. (We are unsure as to the identity of these extra bands.)
HINT3 Antibody - HINT3 was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate. 4 uL anti-HINT3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-HINT3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa.
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Polyclonal Rabbit anti‑Human HINT3 Antibody (IHC, IF, WB) LS‑C816499

Polyclonal Rabbit anti‑Human HINT3 Antibody (IHC, IF, WB) LS‑C816499

Antibody:
HINT3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$412
LS-C816499-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HINT3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HINT3 antibody LS-C816499 is an unconjugated rabbit polyclonal antibody to human HINT3. Validated for ICC, IF, IHC, IP and WB.
Target
Human HINT3
Synonyms
HINT3 | HIT-like protein | HINT4 | HINT-3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human HINT3 fragment
Specificity
Human HINT3
Applications
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HINT3
AA825464

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/28/2024