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Catalog Number Size Price
LS-C804446-100 100 µl (1 mg/ml) $282 
LS-C804446-200 200 µl (1 mg/ml) $350 
HEC1 / NDC80 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
HEC1 / NDC80 Antibody - Western blot analysis of KNTC2 using 293 whole cells lysates
HEC1 / NDC80 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
HEC1 / NDC80 Antibody - Western blot analysis of KNTC2 using 293 whole cells lysates
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Polyclonal Rabbit anti‑Human HEC1 / NDC80 Antibody (IF, WB) LS‑C804446

Polyclonal Rabbit anti‑Human HEC1 / NDC80 Antibody (IF, WB) LS‑C804446

Antibody:
HEC1 / NDC80 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$282
LS-C804446-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HEC1 / NDC80 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
NDC80 antibody LS-C804446 is an unconjugated rabbit polyclonal antibody to NDC80 (HEC1) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
Target
Human HEC1 / NDC80
Synonyms
NDC80 | HEC1 | Kinetochore protein Hec1 | Kinetochore associated 2 | HEC | HsHec1 | HsNDC80 | KNTC2 | TID3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human KNTC2.
Specificity
KNTC2 Antibody detects endogenous levels of total KNTC2.
Applications
  • Immunofluorescence (1:200 - 1:500)
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HEC1 / NDC80
O14777 NM_006101 NP_006092.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 3/27/2025