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Antibodies > HDAC6 > LS-C356091
 
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Catalog Number Size Price
LS-C356091-100 100 µl (1 mg/ml) $315 
HDAC6 Antibody - Immunohistochemical analysis of Histone Deacetylase 6 (pS22) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HDAC6 Antibody - Immunofluorescent analysis of Histone Deacetylase 6 (pS22) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HDAC6 Antibody - Western blot analysis of Histone Deacetylase 6 (pS22) expression in HepG2 (A); HeLa (B) whole cell lysates.
HDAC6 Antibody - Immunohistochemical analysis of Histone Deacetylase 6 (pS22) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HDAC6 Antibody - Immunofluorescent analysis of Histone Deacetylase 6 (pS22) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HDAC6 Antibody - Western blot analysis of Histone Deacetylase 6 (pS22) expression in HepG2 (A); HeLa (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human HDAC6 Antibody (N‑Terminus, IHC, IF, WB) LS‑C356091

Polyclonal Rabbit anti‑Human HDAC6 Antibody (N‑Terminus, IHC, IF, WB) LS‑C356091

Submit Review LSBio Guarantee
Antibody:
HDAC6 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C356091-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HDAC6 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
HDAC6 antibody LS-C356091 is an unconjugated rabbit polyclonal antibody to HDAC6 (N-Terminus) from human. It is reactive with human and monkey. Validated for ICC, IF, IHC and WB.
Target
Human HDAC6
Synonyms
HDAC6 | Histone deacetylase 6 | HD6 | KIAA0901 | JM21
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Histone Deacetylase 6 (pS22).
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of Histone Deacetylase 6 (pS22) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HDAC6
Q9UBN7 NM_006044 NP_006035.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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For technical assistance:
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