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Antibodies > HDAC3 > LS-C800877
 
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Catalog Number Size Price
LS-C800877-100 100 µl (1 mg/ml) $379 
LS-C800877-200 200 µl (1 mg/ml) $421 
HDAC3 Antibody - 1:200 staining human liver carcinoma tissues by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HDAC3 Antibody - 1:50 staining human liver carcinoma tissues by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HDAC3 Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HDAC3 Antibody - Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
HDAC3 Antibody - Western blot analysis of HDAC3 phosphorylation expression in NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
HDAC3 Antibody - Western blot analysis of extracts of rat brain, mouse brain tissue using Phospho-HDAC3 (Ser424) antibody.
HDAC3 Antibody - Western blot analysis of Phospho-HDAC3 (Ser424) antibody expression in mouse lung and mouse brain tissues lysates. The lane on the right is treated with the antigen-specific peptide.
HDAC3 Antibody - Western blot analysis of extracts of rat spleen tissue lysates using Phospho-HDAC3 (Ser424) antibody.
HDAC3 Antibody - 1:200 staining human liver carcinoma tissues by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HDAC3 Antibody - 1:50 staining human liver carcinoma tissues by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HDAC3 Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HDAC3 Antibody - Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
HDAC3 Antibody - Western blot analysis of HDAC3 phosphorylation expression in NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
HDAC3 Antibody - Western blot analysis of extracts of rat brain, mouse brain tissue using Phospho-HDAC3 (Ser424) antibody.
HDAC3 Antibody - Western blot analysis of Phospho-HDAC3 (Ser424) antibody expression in mouse lung and mouse brain tissues lysates. The lane on the right is treated with the antigen-specific peptide.
HDAC3 Antibody - Western blot analysis of extracts of rat spleen tissue lysates using Phospho-HDAC3 (Ser424) antibody.
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Polyclonal Rabbit anti‑Human HDAC3 Antibody (phospho‑Ser424, IHC, IF, WB) LS‑C800877

Polyclonal Rabbit anti‑Human HDAC3 Antibody (phospho‑Ser424, IHC, IF, WB) LS‑C800877

Submit Review LSBio Guarantee
Antibody:
HDAC3 Rabbit anti-Human Polyclonal (pSer424) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800877-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HDAC3 Rabbit anti-Human Polyclonal (pSer424) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HDAC3 antibody LS-C800877 is an unconjugated rabbit polyclonal antibody to HDAC3 (pSer424) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human HDAC3
Synonyms
HDAC3 | RPD3 | SMAP45 | HD3 | Histone deacetylase 3 | RPD3-2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human HDAC3 around the phosphorylation site of Serine 424.
Epitope
pSer424
Specificity
Phospho-HDAC3 (Ser424) Antibody detects endogenous levels of HDAC3 only when phosphorylated at Serine 424.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HDAC3
O15379 NM_003883 NP_003874.2

Publications (0)

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HDAC3 Antibody - Immunohistochemical analysis of Histone Deacetylase 3 (pS424) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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HDAC3 ELISA Kit
Human HDAC3 (Sandwich ELISA) ELISA Kit - LS-F55924
Reactivity: Human
Range: 0.156-10 ng/ml
See all HDAC3 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/6/2025
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Tel: (800) 227-6666 / (650) 697-3600

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