Target
HCV / Hepatitis C Virus
Reactivity
Hepatitis C Virus
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Synthetic peptides derived from HCV capsid protein.
Specificity
Reactive with Recombinant Capsid Protein C and Envelop Protein M (core) (1-142aa) and Synthetic Capsid Protein C (1-61aa). Recognizes different antigenic determinants of HCV capsid protein. Does not cross-react with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).
Applications
- Western blot (0.5 - 1 µg/ml)
- ELISA (1:1)
Usage
Suitable for use in ELISA and Western Blot. ELISA: Indirect ELISA method. The testing plates were coated with HCV Non-Structural Protein (NS), Capsid Protein (C) and the mixture of NS and C separately. The neat culture supernatant of hybridoma showed a strong reaction with HCV C (OD492 value >2) and mixture of NS and C (OD492 value >1.5). Showed no reaction with HCV NS (OD492 value < 0.03). Western Blot: 0.5-1 ug/ml will allow visualization 0.1 ug/lane (Recombinant CPC+EPM (core)), 0.5 ug/lane (Synthetic CPC), and 0.1 ug/lane (Recombinant Chimeric HCV Polyprotein). Testing is under reducing and non-reducing conditions.
Presentation
PBS, pH 7.2, 40% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee