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Antibodies > hCG / Chorionic Gonadotropin > LS-C355430
 
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Catalog Number Size Price
LS-C355430-1 1 mg $801 
hCG / Chorionic Gonadotropin Antibody - Western blot analysis of hCG was performed by loading the indicated amounts of purified hCG protein extracted from human urine, and 15ul of PageRuler Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using a anti hCG antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
hCG / Chorionic Gonadotropin Antibody - Immunoprecipitation of hCG proteins was performed by spiking 5 ugs of purified hCG protein extracted from human urine into 1ml of 1:2 diluted human urine sample. Antigen-antibody complexes were formed by incubating with 5ug of hCG antibody overnight on end over end tube rotator at 4C. The immune complexes were captured by using 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using hCG monoclonal antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
hCG / Chorionic Gonadotropin Antibody - Western blot analysis of hCG was performed by loading the indicated amounts of purified hCG protein extracted from human urine, and 15ul of PageRuler Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using a anti hCG antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
hCG / Chorionic Gonadotropin Antibody - Immunoprecipitation of hCG proteins was performed by spiking 5 ugs of purified hCG protein extracted from human urine into 1ml of 1:2 diluted human urine sample. Antigen-antibody complexes were formed by incubating with 5ug of hCG antibody overnight on end over end tube rotator at 4C. The immune complexes were captured by using 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using hCG monoclonal antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
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Monoclonal Mouse anti‑Human hCG / Chorionic Gonadotropin Antibody (clone SC2, WB) LS‑C355430

Monoclonal Mouse anti‑Human hCG / Chorionic Gonadotropin Antibody (clone SC2, WB) LS‑C355430

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Antibody:
hCG / Chorionic Gonadotropin Mouse anti-Human Monoclonal (SC2) Antibody
Application:
WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$801
LS-C355430-1
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
hCG / Chorionic Gonadotropin Mouse anti-Human Monoclonal (SC2) Antibody
Application:
WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Chorionic Gonadotropin antibody LS-C355430 is an unconjugated mouse monoclonal antibody to human Chorionic Gonadotropin (hCG). Validated for ELISA, IP, RIA and WB.
Target
Human hCG / Chorionic Gonadotropin
Synonyms
hCG
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
SC2
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Human Chorionic Gonadotropin
Specificity
The antibody targets Chorionic Gonadotropin in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects Chorionic Gonadotropin which has a predicted molecular weight of approximately 13 kD.
Applications
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Presentation
0.015 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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hCG / Chorionic Gonadotropin ELISA Kit
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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Vector Laboratories, Inc.
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