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Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Human
(tested or 100% immunogen sequence identity)
Clonality
IgGPolyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1.
Epitope
pSer70
Specificity
The antibody is specific for the phosphorylated form of Hice1. Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Applications
Western blot (1.22 µg/ml)
ELISA (1:17000 - 1:45000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kD in size corresponding to human phosphorylated Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.