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Vector Laboratories, Inc.
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Catalog Number Size Price
LS-C748545-20 20 µl (0.26 mg/ml) $275 
LS-C748545-100 100 µl $389 
HAS3 Antibody - Immunofluorescence analysis of A549 cells using HAS3 antibody. Blue: DAPI for nuclear staining.
HAS3 Antibody - Western blot analysis of extracts of various cell lines, using HAS3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
HAS3 Antibody - Immunofluorescence analysis of A549 cells using HAS3 antibody. Blue: DAPI for nuclear staining.
HAS3 Antibody - Western blot analysis of extracts of various cell lines, using HAS3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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Polyclonal Rabbit anti‑Human HAS3 Antibody (IHC, IF, WB) LS‑C748545

Polyclonal Rabbit anti‑Human HAS3 Antibody (IHC, IF, WB) LS‑C748545

Antibody:
HAS3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C748545-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HAS3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HAS3 antibody LS-C748545 is an unconjugated rabbit polyclonal antibody to HAS3 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human HAS3
Synonyms
HAS3 | Hyaluronan synthase 3 | Hyaluronate synthase 3 | HA synthase 3 | Hyaluronic acid synthase 3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 67-281 of human HAS3 (NP_619515.1). HRRMRRAGQALKLPSPRRGSVALCIAAYQEDPDYLRKCLRSAQRISFPDLKVVMVVDGNRQEDAYMLDIFHEVLGGTEQAGFFVWRSNFHEAGEGETEASLQEGMDRVRDVVRASTFSCIMQKWGGKREVMYTAFKALGDSVDYIQVCDSDTVLDPACTIEMLRVLEEDPQVGGVGGDVQPPGKGMAVEDDQVQAAQVRATEAWSVHQRHVSREQ
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 31kDa/62kDa, while the observed MW by Western blot was 73kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HAS3
O00219 NM_005329 NP_005320.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/26/2024