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Catalog Number Size Price
LS-C313231-10 10 µg $318 
LS-C313231-100 100 µg (0.5 mg/ml) $470 
GSN / Gelsolin Antibody - GSN / Gelsolin antibody. IHC(P): Human Intestinal Cancer Tissue.
GSN / Gelsolin Antibody - GSN / Gelsolin antibody. ICC: NIH3T3 Cell.
GSN / Gelsolin Antibody - GSN / Gelsolin antibody. IHC(P): Rat Cardiac Muscle Tissue.
GSN / Gelsolin Antibody - GSN / Gelsolin antibody. IHC(P): Human Lung Cancer Tissue.
GSN / Gelsolin Antibody - Western blot analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human THP-1 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: monkey COS-7 whole cell lysate, Lane 5: human Caco-2 whole cell lysate, Lane 6: human K562 whole cell lysate, Lane 7: human HL-60 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gelsolin/GSN antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Gelsolin/GSN at approximately 90KD. The expected band size for Gelsolin/GSN is at 86KD.
GSN / Gelsolin Antibody - WB of GSN / Gelsolin antibody. Lane 1: HELA Cell Lysate. Lane 2: A431 Cell Lysate.
GSN / Gelsolin Antibody - GSN / Gelsolin antibody. IHC(P): Human Intestinal Cancer Tissue.
GSN / Gelsolin Antibody - GSN / Gelsolin antibody. ICC: NIH3T3 Cell.
GSN / Gelsolin Antibody - GSN / Gelsolin antibody. IHC(P): Rat Cardiac Muscle Tissue.
GSN / Gelsolin Antibody - GSN / Gelsolin antibody. IHC(P): Human Lung Cancer Tissue.
GSN / Gelsolin Antibody - Western blot analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human THP-1 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: monkey COS-7 whole cell lysate, Lane 5: human Caco-2 whole cell lysate, Lane 6: human K562 whole cell lysate, Lane 7: human HL-60 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gelsolin/GSN antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Gelsolin/GSN at approximately 90KD. The expected band size for Gelsolin/GSN is at 86KD.
GSN / Gelsolin Antibody - WB of GSN / Gelsolin antibody. Lane 1: HELA Cell Lysate. Lane 2: A431 Cell Lysate.
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Polyclonal Rabbit anti‑Human GSN / Gelsolin Antibody (aa763‑775, IHC, WB) LS‑C313231

Polyclonal Rabbit anti‑Human GSN / Gelsolin Antibody (aa763‑775, IHC, WB) LS‑C313231

Note: This antibody replaces LS-C388491
Antibody:
GSN / Gelsolin Rabbit anti-Human Polyclonal (aa763-775) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Monkey, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313231-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
GSN / Gelsolin Rabbit anti-Human Polyclonal (aa763-775) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Monkey, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Gelsolin antibody LS-C313231 is an unconjugated rabbit polyclonal antibody to Gelsolin (GSN) (aa763-775) from human. It is reactive with human, rat and monkey. Validated for ICC, IHC and WB.
Target
Human GSN / Gelsolin
Synonyms
GSN | ADF | AGEL | Brevin | Actin-depolymerizing factor | Gelsolin precursor, plasma | Gelsolin
Host
Rabbit
Reactivity
Human, Monkey, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Gelsolin(763-775 aa WDDDYWSVDPLDR), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Epitope
aa763-775
Specificity
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GSN / Gelsolin
P06396 NM_000177 NP_000168.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/19/2024