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Catalog Number	Size	Price
LS-C344060-10	10 µg	$318
LS-C344060-100	100 µg (0.5 mg/ml)	$470

GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of mouse brain tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

GRIK1 / GLUR5 Antibody - Western blot analysis of GRIK1 using anti-GRIK1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIK1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRIK1 at approximately 104KD. The expected band size for GRIK1 is at 104KD.

GRIK1 / GLUR5 Antibody - GRIK1 antibody Western blot. All lanes: Anti GRIK1 at 0.5 ug/ml. Lane 1: Mouse Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Lane 3: SHG Whole Cell Lysate at 40 ug. Predicted band size: 104 kD. Observed band size: 104 kD.

GRIK1 / GLUR5 Antibody - GRIK1 antibody Western blot. All lanes: Anti GRIK1 at 0.5 ug/ml. WB: Recombinant Human GRIK1 Protein 0.5ng. Predicted band size: 40 kD. Observed band size: 40 kD.

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Polyclonal Rabbit anti‑Human GRIK1 / GLUR5 Antibody (aa271‑450, WB) LS‑C344060

Name: Anti-GRIK1 / GLUR5 Antibody | Rabbit anti-Human Polyclonal WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C344060-10
Price: 318.0000 USD
Availability: InStock

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Note: This antibody replaces LS-C388514

Antibody:

GRIK1 / GLUR5 Rabbit anti-Human Polyclonal (aa271-450) Antibody

Application:

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C344060-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

GRIK1 / GLUR5 Rabbit anti-Human Polyclonal (aa271-450) Antibody

Application:

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

GLUR5 antibody LS-C344060 is an unconjugated rabbit polyclonal antibody to GLUR5 (GRIK1) (aa271-450) from human. It is reactive with human, mouse and rat. Validated for WB.

Target

Human GRIK1 / GLUR5

Synonyms

See All GRIK1 / GLUR5 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human GRIK1 recombinant protein (Position: R271-I450). Human GRIK1 shares 88% and 94% amino acid (aa) sequences identity with mouse and rat GRIK1, respectively.

Epitope

aa271-450

Specificity

Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus.

Applications

Western blot

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About GRIK1 / GLUR5

Uniprot: P39086 NCBI: NM_000830 NP_000821.1