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Catalog Number	Size	Price
LS-C344055-10	10 µg	$318
LS-C344055-100	100 µg (0.5 mg/ml)	$470

GRIA2 / GLUR2 Antibody - GRIA2 antibody IHC-paraffin: Rat Brain Tissue.

GRIA2 / GLUR2 Antibody - GRIA2 antibody IHC-paraffin: Mouse Brain Tissue.

GRIA2 / GLUR2 Antibody - IF analysis of GRIA2 using anti-GRIA2 antibody GRIA2 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-GRIA2 Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

GRIA2 / GLUR2 Antibody - Western blot analysis of GRIA2 using anti-GRIA2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRIA2 at approximately 110KD. The expected band size for GRIA2 is at 99KD.

GRIA2 / GLUR2 Antibody - GRIA2 antibody Western blot. All lanes: Anti GRIA2 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted band size: 99 kD. Observed band size: 99 kD.

GRIA2 / GLUR2 Antibody - Flow Cytometry analysis of U-87MG cells using anti-GRIA2 antibody. Overlay histogram showing U-87MG cells stained with anti-GRIA2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIA2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human GRIA2 / GLUR2 Antibody (aa25‑360, IHC, WB) LS‑C344055

Name: Anti-GRIA2 / GLUR2 Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C344055-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

GRIA2 / GLUR2 Rabbit anti-Human Polyclonal (aa25-360) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C344055-10

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

GRIA2 / GLUR2 Rabbit anti-Human Polyclonal (aa25-360) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

GLUR2 antibody LS-C344055 is an unconjugated rabbit polyclonal antibody to GLUR2 (GRIA2) (aa25-360) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.

Target

Human GRIA2 / GLUR2

Synonyms

See All GRIA2 / GLUR2 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human GRIA2 recombinant protein (Position: N25-I360). Human GRIA2 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA2.

Epitope

aa25-360

Applications

IHC
IHC - Paraffin
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About GRIA2 / GLUR2

Uniprot: P42262 NCBI: NM_000826 NP_000817.2