Target
Mouse GPX4 / MCSP
Synonyms
GPX4 | GPx-4 | MCSP | SnPHGPx | Phospholipid hydroperoxidase | SnGPx | Glutathione peroxidase 4 | GSHPx-4 | PHGPx
Reactivity
Mouse, Rat, Guinea pig
(tested or 100% immunogen sequence identity)
Predicted
Human (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with GPx4 protein from mouse, rat and guinea pig based on 100% homology with the immunizing sequence. Cross-reactivity with human GPx4 is also predicted based on a 94% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
- Western blot (1:500 - 1:3000)
- ELISA (1:7000 - 1:30000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19 kD in size corresponding to GPx4 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee