Rat IgG2a

Goat

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with HRP, HRP.

Purified IgG

Unmodified

Pools of purified homogenous IgG2a isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat IgG2a, subclass specific.

• Immunofluorescence
• ELISA
• Dot Blot

The reactivity of the antiserum is directed to the subclass IgG2a as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to prepare conjugates of the user's own choice. to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.