Rat IgG1

Goat

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Biotin. Also available Unconjugated or conjugated with HRP, FITC, TRITC.

Purified IgG

Unmodified

Pools of purified homogenous IgG1 isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat IgG1, subclass specific.

• IHC
• IHC - Paraffin
• ICC
• ELISA
• Dot Blot
• Immunodiffusion
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the subclass IgG1 as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG1 at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating IgG1 antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of rat origin known to be of the IgG1 isotype in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure IgG1 in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:2000 depending on the method used.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.