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Catalog Number Size Price
LS-C60867-1 1 mg (1 mg/ml) $388 
Rabbit IgG Antibody - Western blot of Biotin conjugated Goat Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Biotin conjugated Goat Anti-Rabbit IgG Pre-Adsorbed at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (Biotin, IHC, WB) LS‑C60867

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (Biotin, IHC, WB) LS‑C60867

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rabbit
Format:
Biotin, Unmodified
Price
Catalog Number
$388
LS-C60867-1
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rabbit
Format:
Biotin, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C60867 is a biotin-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB. Cited in 1 publication.
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:400000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

LSBio Ratings

Goat Anti-Rabbit IgG Secondary Antibody (Biotin) for IHC, WB/Western, ELISA LS-C60867 has an LSBio Rating of
Publications (4)

Publications (1)

Active exercise promotes Achilles tendon healing and is accompanied by the upregulation of collapsin response mediator protein‑2 in rats. Qianman B, Jialihasi A, Asilehan B, Kubai A, Aibek R, Wupuer A, Tangkejie W, Maimaitiaili A, Shawutali N, Badelhan A, Aizezi A, Aisaiding A, Wuerliebieke J, Bakyt Y, Makemutibieke E, Jielile J. Molecular medicine reports. 2017 16:2355-2360. (IHC; Rabbit)

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Requested From: United States
Date Requested: 12/23/2024