Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Immunogen
Mouse IgM whole molecule.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse IgM, and Mouse Serum. Some light chain cross reactivity with Mouse IgG may occur.
Applications
- Immunofluorescence (1:1000 - 1:5000)
- Flow Cytometry (1:500 - 1:2500)
- Fluorophore-Linked Immunosorbent Assay (1:10000 - 1:50000)
Usage
This product is designed for immunofluorescence microscopy and fluorescence based plate assays (FLISA). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee