Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Tetramethyl Rhodamine Iso-Thiocyanate
Purification
Affinity chromatography
Immunogen
Mouse IgG3 heavy chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Applications
- IHC (1:1000 - 1:5000)
- Immunofluorescence (1:1000 - 1:5000)
- Western blot (1:2000 - 1:10000)
- ELISA (1:2000 - 1:10000)
- Fluorophore-Linked Immunosorbent Assay (1:20000 - 1:100000)
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Usage
Anti-Mouse IgG3 secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (ELISA) and fluorescent western blotting as well as other anti IgG3 antibody based assays. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee