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| Catalog Number | Size | Price |
|---|---|---|
| LS-C209483-500 | 500 µg (1 mg/ml) | $472 |
1 of 2
2 of 2
Polyclonal Goat Anti‑Mouse IgG3 Secondary Antibody (Atto 647N, IF, WB) LS‑C209483
Polyclonal Goat Anti‑Mouse IgG3 Secondary Antibody (Atto 647N, IF, WB) LS‑C209483
Antibody:
Mouse IgG3 Goat anti-Mouse Polyclonal (Atto 647N) Antibody
Application:
IF, WB, Flo, FLISA
Reactivity:
Mouse
Format:
Atto 647N, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Mouse IgG3 Goat anti-Mouse Polyclonal (Atto 647N) Antibody
Application:
IF, WB, Flo, FLISA
Reactivity:
Mouse
Format:
Atto 647N, Unmodified
Specifications
Description
Mouse IgG3 antibody LS-C209483 is an Atto 647N-conjugated goat polyclonal antibody. Validated for FLISA, Flow, IF and WB. Cited in 2 publications.
Host
Goat
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Atto 647N
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgG3 heavy chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Applications
- Immunofluorescence (1:5000)
- Western blot (1:4000 - 1:20000)
- Flow Cytometry (1:500 - 1:2500)
- Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Anti-Mouse IgG3 secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
LSBio Ratings
Goat Anti-Mouse IgG3 Secondary Antibody (Atto 647N) for IF/Immunofluorescence, WB/Western, Flow LS-C209483 has an LSBio Rating of
Publications (4.1)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Tolerogenic properties of liver macrophages in non-alcoholic steatohepatitis. Lorenzo A Orci, Mario Kreutzfeldt, Nicolas Goossens, Laura Rubbia-Brandt, Florence Slits, Karim Hammad, Vaihere Delaune, Graziano Oldani, Francesco Negro, Sophie Clément, Carmen Gonelle-Gispert, Léo H Buhler, Christian Toso, Stéphanie Lacotte. Liver international : official journal of the International Association for the Study of the Liver. 2020 March;40:609-621.
Neurons under T Cell Attack Coordinate Phagocyte-Mediated Synaptic Stripping. Giovanni Di Liberto, Stanislav Pantelyushin, Mario Kreutzfeldt, Nicolas Page, Stefano Musardo, Roland Coras, Karin Steinbach, Ilena Vincenti, Bogna Klimek, Thomas Lingner, Gabriela Salinas, Nathalie Lin-Marq, Ori Staszewski, Marta Joana Costa Jordão, Ingrid Wagner, Kristof Egervari, Matthias Mack, Camilla Bellone, Ingmar Blümcke, Marco Prinz, Daniel D Pinschewer, Doron Merkler. Cell. 2018 October;175:458-471.e19.
Customer Reviews (0)
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Species:
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Applications:
IHC, Western blot, ELISA
Species:
Mouse
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry, Fluorophore-Linked Immunosorbent Assay
Species:
Mouse
Applications:
ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/5/2025
Date Requested: 2/5/2025
