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Catalog Number Size Price
LS-C209484-500 500 µg (1 mg/ml) $480 
Mouse IgG1 Antibody - ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
Mouse IgG1 Antibody - Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG1 (gamma 1 chain) Pre-adsorbed secondary antibody. Lane 1: Mouse IgG1. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 647N goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Mouse IgG1 Antibody - ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
Mouse IgG1 Antibody - Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG1 (gamma 1 chain) Pre-adsorbed secondary antibody. Lane 1: Mouse IgG1. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 647N goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Goat Anti‑Mouse IgG1 Secondary Antibody (Atto 647N, IF, WB) LS‑C209484

Polyclonal Goat Anti‑Mouse IgG1 Secondary Antibody (Atto 647N, IF, WB) LS‑C209484

Antibody:
Mouse IgG1 Goat anti-Mouse Polyclonal (Atto 647N) Antibody
Application:
IF, WB, Flo, FLISA
Reactivity:
Mouse
Format:
Atto 647N, Unmodified
Price
Catalog Number
$480
LS-C209484-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Mouse IgG1 Goat anti-Mouse Polyclonal (Atto 647N) Antibody
Application:
IF, WB, Flo, FLISA
Reactivity:
Mouse
Format:
Atto 647N, Unmodified

Specifications

Description
Mouse IgG1 antibody LS-C209484 is an Atto 647N-conjugated goat polyclonal antibody. Validated for FLISA, Flow, IF and WB.
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Atto 647N
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgG1 heavy chain
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Applications
  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • Flow Cytometry (1:500 - 1:2500)
  • Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/17/2024