Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Texas Red.
Also available
Unconjugated or conjugated with
FITC,
HRP,
TRITC,
Biotin,
RPE.
Immunogen
Pooled human myeloma proteins with lambda light chains.
Specificity
Recognizes the beta subunit of cholera toxin. The beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin alpha subunit into cells. The holotoxin consists of a pentameric ring of beta subunits whose central pore is occupied by the alpha subunit. The alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The alpha subunit (and cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.