Target
HRP / Horseradish Peroxidase
Reactivity
Horseradish
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Immunogen
Peroxidase [Horseradish].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Applications
- Western blot (1:900 - 1:4000)
- ELISA (1:9000 - 1:40000)
Usage
This product has been assayed against 1.0 ug of Peroxidase [Horseradish] in a standard sandwich ELISA using Alkaline Phosphatase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and pNPP as a substrate for 30 minutes at room temperature. A working dilution of 1:9000 to 1:40000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Peroxidase [Horseradish] in a standard sandwich ELISA using Alkaline Phosphatase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and pNPP as a substrate for 30 minutes at room temperature. A working dilution of 1:9000 to 1:40000 of the reconstitution concentration is suggested for this product.
Presentation
Lyophilized from 0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee