Reactivity
Guinea pig
(tested or 100% immunogen sequence identity)
Purification
Purified IgG
Immunogen
Purified normal IgG isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Guinea Pig IgG, heavy and light chains.
Applications
- IHC
- IHC - Frozen
- ICC
- Immunofluorescence
- ELISA
- Immunodiffusion
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Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of guinea pig origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:120.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with 2ml dH2O.
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee