Bovine GLUD1/Glutamate Dehydrogenase

Rabbit

Bovine (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and defibrinated

Unmodified

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

• Western blot (1:1000 - 1:3000)
• Immunoprecipitation (1:100)
• ELISA (1:4000 - 1:16000)

This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 56 kD in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 2 mL of deionized water.

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P00367 NCBI: NM_005271 NP_005262.1